Zika virus strain PRVABC59 is propagated in VERO C1008 cells [Vero 76, clone E6, Vero E6] cells (ATCC CRL-1586). This strain was isolated in 2015 from a human serum specimen in Puerto Rico and was deposited by the Centers for Disease Control and Prevention. This viral strain has applications in infectious disease, vector-borne disease, and zoonotic disease research.